NAME read BioRad PIC - Read a Bio-Rad confocal microscope image PIC file into a 3D field. SUMMARY Name read BioRad PIC Type Input Inputs NONE Outputs image - field 3D 3-space 1-vector uniform byte Parameters Name Type Default Choices BioRad PIC File browser File Type Filename XY Pixel Size dial 1.00 0.00 10.00 Z Step Size dial 1.00 0.00 10.00 Header Information textblock Comment Char Text DESCRIPTION This module reads a Bio-Rad confocal microscope image PIC file from disc and outputs a 3D field uniform byte field. The user can specify the pixel size in the xy-direction and the step size between slices in the z-direction. These values are used to set the points coordinates and min/max extents associated with the field. Header information from the file is also displayed. Only 8-bit, non-merged PIC files are read. Notes and look-up tables that are sometimes appended to PIC files are ignored. Further information on the Bio-Rad PIC file format is available from Lance Ladic's 3D Confocal Microscopy page: http://www.cs.ubc.ca/spider/ladic/confocal.html PARAMETERS BioRad PIC File (browser) A file browser that allows the user to specify the image file to be read. XY Pixel Size (dial) Selects the xy-pixel size (in microns) for the image. Z Step Size (dial) Selects the step size (in microns) between slices of the image in the z-direction. Header Information (textblock) A textblock that displays various header values from the file. OUTPUTS image - field 3D 3-space 1-vector uniform byte The output is a 3D uniform field of bytes representing the voxel values in the image file. The coordinates of the voxels in the image range from [0, 0, 0] to [(nx - 1) * XY_Pixel_Size, (ny - 1) * XY_Pixel_Size, (nz - 1) * Z_Step_Size]. The points and min/max extents of the field are set according to these coordinates. EXAMPLE NETWORKS The following network reads a Bio-Rad PIC image file, takes a slice, and then colourizes and displays it: read BioRad PIC | generate colormap orthogonal slicer | | ----------------- | | | colorizer | display image RELATED MODULES write BioRad PIC SEE ALSO http://www.cs.ubc.ca/spider/ladic/confocal.html AUTHOR Chris Pudney (cpudney@alphapharm.pharm.uwa.edu.au) Biomedical Confocal Microscopy Research Centre Department of Pharmacology The University of Western Australia Nedlands, Western AUSTRALIA, 6907.